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Domain organization of N-WASp, autoinhibition by GBD-VCA interaction, multiple effectors (Cdc42, PIP2, Grb2, IcsA) for driving activation of Arp2/3 complex.
Conclusion: Functions and players required for reconstitution of actin-based motility of Listeria or Shigella or any particle. (Slide 17, 18-animation sketch).
Accordingly, branched filaments initiated from ActA-coated beads are attached to the bead and branching points are at equal distance from the ends of the two branches, again suggesting that filaments branch from their barbed ends (Slide 14).
Topology of Arp2/3 complex, localization, activation of polymerization by ActA. (Slide 10)
Physiological activators of Arp2/3 complex : the WASp family. (Slide 11).