Using the motility assay to understand the mechanism of production of force and directional movement
•Control of the concentration of soluble proteins in the motility medium.
•Control of the surface density of filament branching enzyme (N-WASP or ActA).
•Load/velocity relationship: control of the size of the bead and of the viscosity of the medium.
•Frequency of filament branching during movement: two fluorophores (actin and Arp2/3)
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