•Treadmilling of filaments feeds movement
•Functions required for movement:
1)Site-directed generation of barbed ends
by N-WASP (resp. ActA)-activated Arp2/3
2) Chemostat maintaining
a high steady-state concentration of ATP-G-actin : Actin, ADF/cofilin, profilin, Capping protein
•Movement results from a balance between
the creation of new growing
filaments (branching) and death
of these filaments (capping).
Reconstitution of actin-based movement from pure proteins (Loisel et al., Nature
1999)
