WASP family proteins :
activators of Arp2/3 complex
(Miki et al., 1998; Machesky and Insall, 1998; Rohatgi et al., 1999; Egile et al., 1999;
Kim, Rosen et al., 2000; Prehoda et al;, 2000)
R. Robinson et al
 (Science, 2001)
+ X
+ Arp2/3
+G-actin
X
« closed N-WASP »
WH1
GBD
B
VV
C
A
Pro
Plasma membrane
WH1
GBD
B
VV
C
A
Pro
« activated N-WASP »
VV
C
WH1
GBD
A
B
Pr
o
Cdc42
IcsA
TccP
Domain organization of N-WASp, autoinhibition by GBD-VCA interaction, multiple effectors (Cdc42, PIP2, Grb2, IcsA) for driving activation of Arp2/3 complex.