E. coli IcsA
Rate :
≈2 µm/min
Movement of E. coli (IcsA) and Listeria monocytogenes with pure components.
Listeria
Rate :
≈3 µm/min
Essential Proteins :
N-WASP      IcsA-bound
Arp2/3 0.1 µM
Capping Protein   0.1 µM
ADF 2    µM
ATP-actin+F-actin             8    µM
Useful Proteins :
Profilin 2     µM
a-actinin 0.5  µM
VASP                     0.1 µM
Conclusion: Functions and players required for reconstitution of actin-based motility of Listeria or Shigella or any particle. (Slide 17, 18-animation sketch).